Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform.
نویسندگان
چکیده
A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 46 12 شماره
صفحات -
تاریخ انتشار 2008